Genetic diversity and structure assessment of <i>Macrobrachium nipponense</i> populations: implications for the protection and management of genetic resources

This article presents a study of D-loop sequences to characterize the genetic diversity wild Macrobrachium nipponense populations in Yixing natural waters including two reservoirs (Hengshan reservoir, HS; Youche YC), 3 brooks (Linjin Dang, LJD; Magong Dushan MDD; Yangshan YSD) and shallow lakes (Dongjiu lake, DJ; Xijiu XJ; Tuanjiu XJ), compared differentiation population structure with Taihu Lake (TH), Yangtze River (YZ), main local artificially bred varieties “Taihu No. 2” (TH-2). A 747 bp sequence fragment was amplified 321 individuals results exhibited higher content A+T (80.03%) than C+G (19.97%). total 110 haplotypes were identified. The h π value proved these at same level high diversity. TH-2 YZ showed remarkable diversity, XJ is even better. Fst estimates suggested that significant other ( P &lt; 0.05). Three from lake (DJ, TJ) displayed differentiated left ones pairwise distance, as well haplotype network results, also all 11 did not diverge species (&lt;15%). values Tajima's D Fu Fs relatively greater 0.1 &gt; 0.1) nucleotide mismatch distribution analysis multiple peaks, giving conclusion expansion. All have germplasm resources M. are very good suitable for original materials breeding.